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An­ge­bot 101 von 554 vom 14.08.2019, 13:23

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Tech­ni­sche Uni­ver­sität Ber­lin - Fac­ulty III - Insti­tute of Bio­tech­no­logy / Chair of Bioana­lyt­ics

Research Assist­ant - 0.67 work­ing time - salary grade E13 TV-L Ber­liner Hoch­schu­len

under the reserve that funds are gran­ted

Work­ing field:

The focus of the DFG-fun­ded pro­ject "Site Spe­cific Cross­link­ing with KLK Pro­teases from the Pro­state" (BU 1404 12-1) is on the devel­op­ment of meth­ods for the detec­tion and iden­ti­fic­a­tion of short-lived pro­tein-lig­and com­plexes dif­fi­cult to access with clas­sical chem­ical modi­fic­a­tions. In par­tic­u­lar, non-canon­ical amino acid (ncAAs) based click chem­istry will be used to study pro­tein-lig­and inter­ac­tions in human kal­likrein-related pep­ti­dases (KLKs) from the pro­state (espe­cially KLKs 2, 3, 4). The main task is to develop a site-spe­cific / stat­ist­ical cross-link­ing of pro­teins with ncAAs, which should gen­er­ally be applic­able to pro­tein com­plexes with dif­fer­ent lig­ands. The spe­cific goal is to determ­ine enzymatic sub­strate spe­cificity (in the con­crete case, "ter­tiary spe­cificity" of the KLK pro­teases) use­ful for elu­cid­at­ing bio­lo­gical and dis­ease-related pro­cesses, e.g. con­trib­ute to pro­state can­cer and allow the design of new drugs. Par­al­lel to work with recom­bin­ant KLK pro­tease and chem­ical syn­thesis of desired ncAAs, the chem­ical syn­thesis of suit­able lig­ands will be per­formed as well. Basic ana­lyt­ical (amino acid ana­lysis, vari­ous mass spec­tro­met­ric meth­ods, etc.) and bio­phys­ical meth­ods (NMR, CD spec­tro­scopy, fluor­es­cence and UV absorb­ance, etc.) find a very strong applic­a­tion throughout the pro­ject. Bio­syn­thetic work includes enzyme evol­u­tion for novel sub­strates, design of ortho­gonal pairs, estab­lish­ment of ortho­gonal pairs in bac­terial and euk­a­ryotic cells, and sub­sequent optim­iz­a­tion of expres­sion and meas­ure­ment con­di­tions. The incor­por­a­tion of the ncAAs will be car­ried out by means of vari­ous ortho­gonal pairs or in com­bin­a­tion with the selec­tion pres­sure incor­por­a­tion (SPI) method (stat­ist­ical and site-dir­ec­ted labelling).

Require­ments:

Suc­cess­fully com­pleted uni­versity degree (Mas­ter, Dip­lom or equi­val­ent) prefer­ably in chem­istry, bio­tech­no­logy, chem­ical bio­logy or a related field; a very good grade aver­age desired. Can­did­ates who are about to gradu­ate are also invited to apply. The gen­er­a­tion of suit­able expres­sion sys­tems requires extens­ive know­ledge in the field of molecu­lar bio­logy (clon­ing meth­ods, clon­ing soft­ware such as Geneious / Clone Man­ager / APE, expres­sion vec­tors and strains). Pro­tein and pep­tide puri­fic­a­tion require very good know­ledge of the com­mon chro­ma­to­graphic meth­ods (see above) and the hand­ling of the Äkta sys­tems avail­able at the insti­tute. For the inter­pret­a­tion of u. a. MS spec­tra, activ­ity and fluor­es­cence assays require good com­puter skills in the area of data ana­lysis (Excel, Ori­gin / SigmaPlot) and pro­tein struc­ture ana­lysis (Pymol / Chi­mera / Swiss PDB viewer or sim­ilar). Exper­i­ence in the ana­lysis of pro­teins by means of cross­link­ing mass spec­tro­metry as well as in chem­ical syn­thesis of amino acids includ­ing pep­tide syn­thesis is espe­cially advant­age­ous.
The capa­city to com­mu­nic­ate with vari­ous spe­cial­ists in the fields of syn­thetic chem­istry, molecu­lar bio­logy and bio-medi­cine is a pre­requis­ite. Open­ness for inter­dis­cip­lin­ary research pro­jects in the field of nat­ural sci­ences and high motiv­a­tion for inde­pend­ent sci­entific work ideally with (if pos­sible) proven suc­cess through pub­lic­a­tions; very good com­mu­nic­a­tion and team skills and excel­lent com­mand of spoken and writ­ten Eng­lish.

How to ap­ply:

Please send your writ­ten applic­a­tion with the ref­er­ence num­ber and the usual doc­u­ments to Tech­nis­che Uni­versität Ber­lin - Der Präsid­ent - Fakultät III, Institut für Biotechnologie, FG Bioanalytik, Prof. Dr. Rappsilber, Sekr. TIB 4/4-3, Gustav-Meyer-Allee 25, 13355 Berlin or by e-mail to h.weiss@tu-berlin.de.

To ensure equal opportunities between women and men, applications by women with the required qualifications are explicitly desired. Qualified individuals with disabilities will be favored. The TU Berlin values the diversity of its members and is committed to the goals of equal opportunities.

Please send copies only. Original documents will not be returned.