12 PhD positions in the Research Training Group “TJ-Train” (GRK2318)
“TJ-Train” offers 10 PhD positions at Charité, 1 PhD position at Max Delbrück Center for Molecular Medicine (MDC) Berlin, and 1 PhD position at Leibniz-Institute for Molecular Pharmacology (FMP) Berlin
The Research Training Group TJ-Train aims at analyzing different aspects of tight junctions in 3 distinct project areas.
The tight junction (TJ) connects neighbouring epithelial or endothelial cells and is the central element of barrier function, as it controls paracellular passage of solutes and water. The TJ protein composition varies greatly in different tissues and determines the tightness and selective permeability. TJs underlie extensive extra- and intracellular signaling, but also regulate epithelial functions and cellular differentiation. Furthermore, numerous TJ proteins serve as receptors for certain pathogens. Thus, TJ proteins are involved in the pathogenesis of numerous diseases. Alterations in TJ composition and architecture may cause for instance unwanted leak flux or antigen passage and may thus maintain disease activity or even directly trigger disease onset.
Project area A is focused on basic research on structure and function of TJ proteins, which provides the requirement for understanding TJ development and regulation and its role in pathogenesis of diseases. The investigations on structure and function of TJ molecules comprise state-of-the-art molecular biological techniques, advanced electrophysiological methods, water transport measurements, and super resolution microscopy.
A1 Impedance spectroscopy of epithelial barrier properties
A2 Structure-based modification of CPE to target claudins
A3 Claudin- and TAMP-mediated water transport
A4 High- and super-resolution imaging of tight junction components
Project area B focuses on the role of TJ proteins in the developing tissue and the regulation of TJ proteins and TJ assembly on different levels, e.g. transcriptional regulation, interaction of different pathways, and genetic predisposition. Through the projects of area B students will get competent knowledge of transgene technology, genotyping and phenotyping of mouse models, and methods in next generation sequencing.
B1 Neuroepithelial barrier function in brain development
B2 Tracking a primary barrier defect in celiac disease
B3 The magnesiome, an integrative view of magnesium regulation
B4 Transcriptional integration of TJ assembly in renal epithelial cells
Project area C focuses on modulation of TJ in intestinal disease associated with barrier dysfunction. Major aim is the understanding of TJ modulation in infectious and inflammatory based intestinal disease and the identification of barrier-associated therapeutic targets. New confocal visualization techniques for macromolecule passage across epithelia and for cyst- or organoid-based cell culture models are implemented in project area C.
C1 Toxin effects on paracellular intestinal barrier function
C2 Role of the tricellular TJ in inflammatory bowel diseases (IBD)
C3 Epithelial dyspolarity in chronic intestinal inflammation
C4 Macromolecule uptake across the mucosa in HIV infection
very good University degree (equivalent diploma/master) in biology, biochemistry, biotechnology or a related subject
strong scientific motivation
special interest in cell biology, functional and pathophysiological-translational issues
very good verbal and written English communication skills
participation in the qualification program of the Research Training Group
What we offer:
working at the Charité, one of the largest university hospitals in Europe, a joint institution of Humboldt-Universität zu Berlin and Freie Universität Berlin, and at famous research institutes in Berlin, MDC and FMP
research in a world-renowned center in the field of tight junction research
an excellent scientific environment at the interface between basic and preclinical research
doctoral thesis associated with an excellent qualification program
fixed-termed employment contract (65% E13, dependent on personal qualification) limited to 3 years
How to apply:
For any further information concerning requirements and application process please contact Rita Rosenthal (email@example.com) and access the link below to find out more information: https://klinphys.charite.de/grk/
Please submit your application via email to firstname.lastname@example.org. The application will consist of one pdf file which includes application form, CV, all transcripts, certificates, and the reference letters of two academic teachers/supervisors.